Organic Beta Carotene Oil is a reddish-brown liquid and is manufactured by micronization technology. The micronized beta-carotene crystals are evenly dispersed in sunflower oil. DL-Alpha-Tocopherol is added as antioxidant. The beta-carotene crystal is obtained by a fermentation process using a mixed culture of the two sexual mating types(+) and (-) of natural strains of the fungus Blakeslea Trispora. The crystallised products consist mainly of trans beta-carotene. Used as a coloring agent or nutrient supplement for oil-based foods like margarine and soft capsule preparations.

Chemical Structure

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Chemical Formula: C40H56 Molecular Weight: 536.85

Product Specifications

Light absorption(in cyclohexane)	Red-brown liquid
Maximum absorption	At 453-457nm
Absorption at 340nm	≤0.080
Heavy metals	≤10ppm
Lead	≤3ppm
Arsenic	≤1ppm
Cadmium	≤0.1ppm
Mercury	≤0.1ppm
Assay	≥30.0%
Total aerobic count	≤1000cfu/g
Yeats & Moulds	≤100cfu/g
E-Coli	≤30 MPN/100g
Salmonella	Negative
Staphylococcus aureus	Negative

Nutrition information

Items	Unit	Value
Energy	kJ	3100
Protein	g	0
Total fat	g	70.0
Carbohydrate	g	30.0
Sodium	mg	0

Ingredients Sheet

Ingredients	Composition
Beta-Carotene Pure	30.0%
Sunflower Oil	67.0%
Dl-Alpha-Tocopherol	3.0%

Product Advantages

Natural Colorant: beta-carotene liquid provides a vibrant orange hue, making it an ideal natural pigment for food and cosmetic applications, replacing synthetic dyes.

Antioxidant Properties: This oil is rich in antioxidants, which help combat oxidative stress and may reduce the risk of chronic diseases.

Supports Eye Health: As a precursor to vitamin A, nature-made beta carotene plays a crucial role in maintaining vision and eye health.

Enhances Immune Function: Regular consumption can help strengthen the immune system, supporting your body's natural defenses.

Versatile Applications: Suitable for use in food products, cosmetics, dietary supplements, and animal feed, making it a highly adaptable ingredient.

Technical Features

Screening and Optimization of Natural Strains:

Strain Library Development: A specialized microbial repository was established by assembling over 3,000 natural microbial strains.

High-Throughput Screening: Microfluidic chip technology enabled rapid evaluation of approximately 500 strains daily. This process identified Blakeslea trispora HSF-Bt07, a strain exhibiting β-carotene production capacity threefold higher than wild-type counterparts.

Adaptive Evolution (ALE): Through prolonged stress cultivation (e.g., high osmotic pressure, low temperatures), the strain's robustness was enhanced. Post 20 successive fermentation cycles, the strain maintained 99% stability with no yield decline.

Closed-Loop Intelligent Fermentation System:

Light/Oxygen Sensitivity Mitigation: Dual-layer stainless steel fermentation tanks with UV-blocking coatings and integrated nitrogen blanketing systems minimized light/oxygen exposure. Dissolved oxygen (DO) levels were maintained at ≤3% (vs. industry average 8%).

Dynamic Process Control:

Real-Time Monitoring: Embedded sensors tracked 12 parameters (pH, temperature, cell density, etc.) at 10-second intervals.

AI-Driven Feeding: Machine learning algorithms predicted carbon-to-nitrogen (C/N) demands, enabling automated feed rate adjustments. This boosted cell density to 65 g DCW/L (vs. 40 g DCW/L in conventional methods).

Two-Phase Fermentation Strategy:

High-Density Growth Phase: Optimized nutrient formulations accelerated biomass accumulation by 300% within 48 hours.

Productivity Phase: Gradual temperature increases triggered metabolic surges, achieving β-carotene yields of 10 g/L while shortening the fermentation cycle.

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Organic Certification and Regulatory Compliance

Certified Organic Production Under EU and COSMOS Standards

For organic-certified grades, the production process excludes synthetic solvents, petrochemical-derived processing aids, and prohibited additives. The formulation uses dl-alpha-tocopherol as the antioxidant, derived from non-GMO vegetable sources. The product holds EU Organic certification and is compliant with COSMOS standards for natural cosmetic applications, meeting the requirements of Regulation (EU) 2018/848 for organic production.

This certification positions the product for use in organic-certified food products and natural cosmetic formulations where synthetic colorants and conventional processing aids are not permitted.

Solvent-Free Extraction and Purification

Green Process Design

The extraction and purification of beta-carotene from the fermentation biomass is conducted without organic solvents. The process operates under mild conditions (35°C and 0.6 MPa) to preserve the trans-isomer configuration of the carotenoid. Solvent recovery is achieved through reduced-pressure evaporation, with a recycling rate of 98%.

The resulting product consists primarily of trans beta-carotene, as confirmed by light absorption analysis at 453–457 nm. This isomer profile is consistent with the naturally occurring form found in food sources.

3. Patent Portfolio and Technical Foundation

Proprietary Technologies Supporting the Product Line

HSF holds 72 patents covering natural vitamin E, phytosterols, beta-carotene, micro-encapsulation powders, and natural colors. The strain development and fermentation processes underlying this product are protected by proprietary technologies including the HSF-Bt07 strain and the adaptive evolution methodology described in the existing Technical Features section.

The R&D Center operates core platforms for strain library construction, high-throughput screening, protein engineering, and fully automated fermentation, providing ongoing technical support for product quality and supply consistency

Product Applications

Beta beta-carotene oil is incredibly versatile and can be used in various industries:

Food Industry: Add color and nutrition to beverages, dairy products, sauces, and candies.
Cosmetics and Personal Care: Perfect for skin care creams, lotions, and hair products, enhancing both appearance and effectiveness.
Nutritional Supplements: Used in capsules and powders to boost vitamin A intake, catering to health-conscious consumers.
Animal Feed: Enhances the color and nutritional value of poultry, fish, and livestock feed, promoting healthier animals.

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R&D Infrastructure and Innovation Platform

Vertically Integrated Development Pipeline

The technical capabilities behind this product are supported by the HSF R&D Center, which operates core platforms for proprietary strain library construction, high-throughput screening, protein engineering, and fully automated fermentation. The center maintains a comprehensive microbial strain discovery and optimization platform alongside a microbial precision fully automated fermentation platform.

Provincial Innovation Leadership

In 2024, HSF led the establishment of the Biosynthesis Innovation Center of Shaanxi Province, approved by the Shaanxi Provincial Department of Industry and Information Technology. The center was jointly established with China Agricultural University, Shaanxi Normal University, Beijing University of Chemical Technology, and other institutions, leveraging the technical support of the National Key Laboratory of Green Biomanufacturing.

This platform operates a full-chain innovation system covering cell factory R&D, enzyme engineering R&D, pilot process development, functional services, and operation management-a structure designed to accelerate the translation of laboratory results into commercial-scale production.

HSF R&D Center

With its capability to advance projects from gene-level pathway engineering through to commercial-scale fermentation, HSF R&D Center offers a complete technological pipeline supported by core platforms for strain library construction, high-throughput screening, and automated process control.

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