β-Carotene (C40H56) is one of the carotenoids, an orange-yellow lipid-soluble compound that is the most common and stable natural pigment in nature.

HSF's Natural Beta-carotene 96% Powder/Crystalloid is a kind of red to brownish-red crystalline powder based on the fermentation of Blakeslea trispora. β-Carotene is widely used in the food, feed, pharmaceutical, and cosmetic industries.

The metabolism of Blakeslea trispora can produce natural beta-carotene powder and lycopene. The performance of this strain has been widely recognized and industrialized. The pigments produced by the fermentation method of Blakeslea trispora have the characteristics of natural products, short cycle, and high yield. It has been technologically advanced and solved the problems of seasonal shortage of raw materials and high cost of pigments obtained by plant extraction methods.

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Synonyms	beta,beta-Carotene; Carotene; Solatene; trans-beta-Carotene
Molecular formula	C40H56
Molecular weight	536.88
Assay	96.0%-101.0%
Quality Standards	USP30
Characteristic	Dark red or dark red, shiny side of the ramp hexahedral or crystalline powder
Melting point	183°C
Solubility	Soluble in carbon disulfide, benzene, and chloroform. It does not dissolve in water, propylene glycol, or glycerin and is almost insoluble in methanol and ethanol.

Ingredients Sheet

Ingredients	Composition
Beta Carotene	≥ 96%
Carotenoids other than Beta-carotene	≤ 3.0%
Moisture and Ash	≤ 1.0%

Specification

Physical & Chemical Data
Color	Red to brownish-red
Appearance	Crystalline powder
Identification	Spectrometry Maximum in cyclohexane at 453 nm to 456 nm
Analytical Quality
Content of Beta Carotene	≥ 96%
Sulphated Ash	≤ 0.2%
Absorbance Ratio
A455/A483	1.14- 1.19
A455/A340	≥ 0.75
Contaminate
Lead	≤ 2.0ppm
Arsenic	≤ 3.0ppm
Residual Solvents
Ethyl Acetate	≤ 0.8% singly or combination
Ethanol
Microbiological
Total Aerobic Microbial Count	≤ 1000cfu/g
Molds	≤ 100cfu/g
Yeasts	≤ 100cfu/g
Salmonella spp.	Negative/25g
Escherichia coli	Negative/5g

Microbial Strain Development & Smart Fermentation System

1. Strain Screening & Optimization

Built a 3,000+ microbial strain library.

High-throughput screening via microfluidic chips (500 strains/day) identified Blakeslea trispora HSF-Bt07 with 3× higher β-carotene yield than wild strains.

Adaptive evolution (ALE) under stress (high osmotic pressure/low temperature) enhanced stability: 99% yield retention over 20 generations.

2. Light/Oxygen-Sensitive Fermentation System

Double-layer stainless steel bioreactor:

UV-resistant coating + nitrogen blanketing → dissolved oxygen ≤3% (vs. industry average 8%).

3. Dynamic control technology

Embedded sensors collect 12 parameters (pH, temperature, cell density) every 10s.

Machine learning-based feeding adjusts C/N ratio → cell density reaches 65g DCW/L (vs. 40g in traditional processes).

Two-Phase Fermentation:

48h biomass growth phase: 300% biomass increase.

Metabolic burst phase: Gradient temperature triggers β-carotene production at 10g/L.

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High-Concentration Crystalloid

The product is supplied as a high-purity crystalloid/powder with a standardized beta-carotene content of ≥96%. This high concentration allows formulators to achieve target dosages with lower inclusion rates, reducing carrier or excipient load in downstream applications such as soft capsules, tablets, and fortified foods.

Downstream Purification and Residual Solvent Control

After fermentation, the product undergoes cell disruption, solvent extraction, chilling crystallization, and vacuum drying. Residual solvent levels are controlled to ethyl acetate ≤0.8% and ethanol ≤0.8%, consistent with ICH Q3C and USP30 requirements. The purification process is designed to preserve the natural cis/trans isomer profile while removing impurities.

Formulation Flexibility from a High-Potency Intermediate

As a high-concentration intermediate, the crystalloid can be further processed into oil suspensions, water-dispersible beadlets, or microencapsulated powders. This gives formulators flexibility across both oil- and water-based systems, while supporting cost-efficient use in applications where high dosage precision is required.

Quality Consistency and Technical Support

Each batch is assayed by HPLC and UV spectrophotometry, with absorbance ratios such as A455/A483 and A455/A340 used to verify identity and isomer consistency. The product is supported by HSF's R&D infrastructure for strain development, high-throughput screening, and pilot-scale process development. Technical documentation, including TDS, stability data, and customized formulation support, is available upon request.

Flow Chart

Why Choose HSF?

1. Innovation-driven Biotechnology Company: Focused on the development of patented natural products with 22 patented technologies.

2. International Certification System: Passed ISO9001, ISO22000, IP(non-GMO), FAMI-QS, Kosher, Halal, and Organic etc.

3. Professional and Experienced R&D Team: Professors from prestigious universities and experts with decades of working experience in this industry.

4. Supply Diversified Products: Products have been applied in all main international brands within the pharmaceuticals, dietary supplements, cosmetics, animal nutrition, and functional food sectors.

HSF

Relying on advanced technology, HSF is equipped with modern production equipment, follows scientific production processes, produces strictly according to international production technical standards, and provides customers with high-performance and high-quality Natural Beta-carotene 96% Powder/ Crystalloid with excellent service.

HSF R&D Center

With its capability to advance projects from gene-level pathway engineering through to commercial-scale fermentation, HSF R&D Center offers a complete technological pipeline supported by core platforms for strain library construction, high-throughput screening, and automated process control.

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