1. Product introduction
HSF LowChol-E Phytosterol Ester is a pale yellow oily paste at room temperature, and will be clear oily liquid above 80ºC/176°F. It is a green process that uses neither catalysts nor solvents, produced by esterification of free plant sterols with fatty acids.
This green process through biotransformation technology and low temperature separation technology retains the biological activity of the natural functional components of phytosterol ester, making the product more stable and more convenient.
In order to meet the needs of customers and markets in different fields, HSF Biotech has successfully developed more than 10 plant sterol ester series products. After years of technological innovation and public relations, it has been widely used in edible oils, special oils, dairy products (dry, wet), functional foods, solid beverages, cosmetics and daily chemical products.
1.1 Chemical structure
Chemical Formula | Molecular Weight | |
Beta-sitosterol oletate | C47H82O2 | 678 |
Stigmasterol oletate | C47H80O2 | 676 |
Campesterol oletate | C46H80O2 | 664 |
Brassicasterol oletate | C46H78O2 | 662 |
1.2 Physical and chemical properties
Product Snapshot | |
Specification | 97% |
Odor | Slightly vegetable oil |
Taste | Little to none |
Appearance | Light yellow oily paste (at ambient temperature) |
Transparency(80℃) | Clear oily liquid |
Recommended Use | Food/Pet food/Cosmetics additive |
Solubility | Insoluble in water (20℃) Soluble in fats and oils |
2. Benefits of Esterified Phytosterols
2.1 The phytosterol ester obtained by esterification can greatly improve the fat-solubility or water-solubility, so that it can be added to oily or water-containing foods.
2.2 The esterified phytosterols are more soluble in organic solvents, and the absorption and utilization rate is about 5 times higher than that of the free type.
There are two types of phytosterols in nature: free type and esterified type. The free type is mainly in nuts and oil crops, and the esterified type is mainly in cereals. Free phytosterols in the form of crystals are difficult to absorb in the human body due to their insolubility and low bioavailability, and require higher doses to be effective, which can easily lead to hyperphytosterolemia.
3. Applications
The product can be applied in food field, health product field,cosmetics field and pharmaceutical field.
4. Flow Chart

5. Quality Standard
Analytical Quality | Test Methods | |
Acid Value | ≤ 1.0mg KOH/g | USP<401> |
Peroxide Value | ≤ 5.0meq/kg | USP<401> |
Loss on Drying | ≤ 0.2% | USP<731> |
Assay | ||
Content of Sterol Esters & Free Sterols | ≥ 97% | GC |
Sterol Esters | ≥ 90% | GC |
Free Sterols | ≤ 6% | GC |
Total Sterols | ≥ 59% | GC |
Contaminant | ||
Pb | ≤ 0.1ppm | GF-AAS |
As | ≤ 0.1ppm | HG-AAS |
Cd | ≤ 1.0ppm | GF-AAS |
Hg | ≤ 0.1ppm | HG-AAS |
B(a)p | ≤ 2.0ppb | HPLC |
Residual Solvents | ≤ 50ppm | USP<467> |
Microbiological | ||
Total Aerobic Microbial Count | ≤ 1000cfu/g | USP<61> |
Total Yeasts and Molds Count | ≤ 100cfu/g | USP<61> |
E. coli | Negative/10g | USP<62> |
6. Nutrition Information
6.1 Specification: Plant Sterol Ester (Soy)
Per 100g
Items | Unit | Value |
Energy | kJ | 3684 |
Protein | g | 0 |
Total fat | g | 99.1 |
Carbohydrate | g | 0.8 |
Sodium | mg | 0 |
6.2 Specification: Plant Sterol Ester (Pine)
Per 100g
Items | Unit | Value |
Energy | kJ | 3689 |
Protein | g | 0 |
Total fat | g | 99.4 |
Carbohydrate | g | 0.8 |
Sodium | mg | 0 |
7. Ingredients Sheet
Specification: Phytosterol Esters 97%
Ingredients | Composition |
Sterol ester & Free sterol | ≥ 97% |
Neutral fatty acid glyceride and Ash | ≤ 3% |
8. Test Equipment

9. Product Qualification
Certificates such as ISO9001, ISO22000, FAMI-QS, IP(NON-GMO), Kosher, Halal, cGMP are in place.

10. Patent Title

HSF Biotech participated in the formulation of the industry standard for phytosterol ester in China. It is the earliest enterprise engaged in the research, development and industrialized production of phytosterol ester in China. In addition, we have a number of invention patents for phytosterol esters.
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